The early allergic response in small airways of human precision-cut lung slices
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چکیده
منابع مشابه
The early allergic response in small airways of human precision-cut lung slices.
To study the role of small airways in the early allergic response (EAR), the method of human precision-cut lung slices (PCLS) was developed and used to examine the bronchoconstriction elicited by passive sensitisation and allergen provocation. Viable human PCLS of 250-microm thickness containing airways <1.5 mm in outer diameter were prepared from lung lobes obtained from lung resection and tak...
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The mechanical forces acting on lung parenchyma during (mechanical) ventilation and its (patho)physiological consequences are currently under intense scrutiny. Several in vivo and cell culture models have been developed to study the pulmonary responses to mechanical stretch. While providing extremely useful information, these models do also suffer from limitations in being either too complex fo...
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Contraction of airways of different size can be studied in viable lung slices by videomicroscopy. However, at present, application of this technique is limited by the heterogeneous responses obtained. We investigated the use of precision-cut lung slices to examine contraction of individual airways. Lung slices of 250 +/- 20 microns were prepared from Wistar rats and cultured in a roller incubat...
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Rosiglitazone elicits in vitro relaxation in airways and precision cut lung slices from a 1 mouse model of chronic allergic airways disease 2 3 1, Chantal Donovan, Simon R Bailey, Jenny Tran, Gertruud Haitsma, Zaridatul A 4 Ibrahim, Simon R Foster, Mimi L K Tang, 1,2,4+ Simon G Royce, Jane E Bourke 5 6 Biomedicine Discovery Institute and Department of Pharmacology, Monash University, 7 Clayton,...
متن کاملRosiglitazone elicits in vitro relaxation in airways and precision cut lung slices from a mouse model of chronic allergic airways disease.
Rosiglitazone (RGZ), a peroxisome proliferator-activated receptor-γ (PPARγ) ligand, is a novel dilator of small airways in mouse precision cut lung slices (PCLS). In this study, relaxation to RGZ and β-adrenoceptor agonists were compared in trachea from naïve mice and guinea pigs and trachea and PCLS from a mouse model of chronic allergic airways disease (AAD). Airways were precontracted with m...
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ژورنال
عنوان ژورنال: European Respiratory Journal
سال: 2003
ISSN: 0903-1936,1399-3003
DOI: 10.1183/09031936.03.00027502